Experiment code 19.3.3.19
Experiment Title Isolation and identification of microbial diversity from Jeevamrut and their antagonistic effect against soil borne pathogens
Research Type Departmental Research
Experiment Background Liquid organic formulations like Panchagvya, Beejamrut and Jeevamrut are used in natural farming. These arerich sources of beneficial microflora which support, stimulate the plant growth, better vegetative growth and ,alsoobtaine good quality yield (Devakumaret al.,2014). Devakumaret al. (2008) and Srinivas et al. (2010) have reported the presence of many beneficial microorganisms viz., nitrogen fixers, phosphorus solubilizers, actinomycetes and fungi in Jeevamrut and Beejamrut. Swaminathan (2005) showed that naturally occurring beneficial microorganisms mainly bacteria, yeasts, actinomycetes, photosynthetic bacteria and certain fungi were detected in cow dung which is one of component of Jeevamrut, contain macro as well as micro nutrients, many vitamins, essential amino acids, growth promoting factors like Indole Acetic Acid(IAA),Gibberellic acid (GA) and beneficial microorganisms (Natrajan 2007,Sreenivasa 2010). Maximum colonies of bacteria, fungi, actinomycets, N-fixers and P-solubilizers were present in of beneficial microorganisms in jeevamrut formulation might be mainly due to their constituents such as: cow dung, cow urine, legume flour and jaggery and associated microorganisms and their products (Palekar, 2006; Sreenivasaet al., 2010).jeevamrut was made following serial dilution and plate count technique. Samples were drawn on a daily basis up to 7 days after preparation (DAP) for up to 20 days for Jeevamrut. Samples were studied for five groups of micro organisms’ viz., bacteria & fungi, actinomycets, N-fixers and P-solubilizers. The different fungi observed were: Aspergillus sp., Fusarium sp., Penicillium sp., Trichoderma sp., isolated P-solubilisers fungi like - Aspergillus sp., Penicillium sp., Bacteria like- Bacillus sp., Pseudomonas sp., and N-fixers like bacteria - Azatobacter sp., A.chroococcum, Bacillus sp., Beijerinckia sp., Actinomycetes -Streptomyces sp. The presence of beneficial microorganisms in organic liquid formulation might be mainly due to their constituents such as: cow dung, cow urine, legume flour, handful soil from banyans tree and jaggery and associated microorganisms and their products (Palekar, 2006; Sreenivasaet al., 2010). It clearly indicates that the jeevamrut is enriched consortia of native soil microorganisms. The preparation would give the best results if it is used between 9th to 12th days after preparation. Hence, this experiment will be conducted for finding and identify most out effective bioagents from jeevamrut.
Experiment Group Plant Pathology
Unit Type (02)EDUCATION UNIT
Unit (12)NAVINCHANDRA MAFATLAL COLLEGE OF AGRICULTURE (NAVSARI)
Department (263)Plant Pathology, NMCA, Navsari
BudgetHead (303/12243/00)303/03/REG/01792
Objective
  1. Isolation and identification of microorganisms from Jeevamrut
  2. In vitro efficacy of isolated microorganisms against soil borne pathogens
Season -
Location Unit Type (02)EDUCATION UNIT
Location Unit (12)NAVINCHANDRA MAFATLAL COLLEGE OF AGRICULTURE (NAVSARI)
Location Department (263)Plant Pathology, NMCA, Navsari
Plot No Laboratory study
PI Name (NAU-EMP-1995-000237)KALUBHAI BALUBHAI RAKHOLIYA
PI Email rakholiyakb@nau.in
PI Mobile 9426213756
Year of Approval 2023
Commencement Year 2023
Completion Year 2025
Design of Experiment

CRD

Crop Spacing (cm x cm)

NA

Gross Plot (m x m) NA
Net Plot (m x m) NA
Total Experiment Area (m2) NA
Plot History Last Three Year

NA

Initial Soil Sample Analysis Report

NA

Initial Soil Sample Analysis Report Attachment Attachment Not Available!
Layout Plan

NA

Layout Plan Attachment Attachment Not Available!
Treatment

Experimental details

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Different microbes will be isolated on following general purpose or specific medium

Treatments

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Treatment details

 

 

 

Tr. No.

Treatments

 

 

 

T1

Nutrient agar medium

 

 

 

T 2

Potato dextrose agar medium

 

 

 

T3

Actinomycetes specific medium

 

 

 

T4

Yeast specific medium

 

 

 

T5

Jeevamrut medium with agar

 

 

 

T6

Trichoderma specific medium

 

 

 

T7

Ashby’smannitol  agar medium

 

 

 

T8

King’s medium

 

Treatment Attachment Attachment Not Available!
(NAU-EMP-2011-000351) MRUGESHKUMAR DHIRAJLAL KHUNT khunt_mrugesh@nau.in 9427731326 16/02/2024
Active
(NAU-EMP-2015-000325) SHIVANGI SUNILKUMAR KANSARA shivangi.kansara@nau.in 9687545952 16/02/2024
Active
(NAU-EMP-2015-000215) GOPALKUMAR BABUBHAI CHOPADA chopada.gopal@nau.in 9687412003 16/02/2024
Active
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