| Experiment code | 20.6.3.14 | ||
|---|---|---|---|
| Experiment Title | Molecular barcoding of Spodoptera frugiperda affecting sorghum crop | ||
| Research Type | Departmental Research | ||
| Experiment Background | Studies on population genetics of agricultural pests have emphasized the importance of the genetic structure and patterns of gene flow for the effective implementation of Integrated Pest Management (IPM) programs (Krafsur, 2005; Ochando et al., 2010) and for implementing strategies for insect resistance management to insecticides and transgenic crops (Gould et al., 1995; Fuentes-Contreras et al., 2008). Although gene flow and genetic variation among populations may be difficult to measure directly, these parameters can be estimated by using molecular markers (Slatkin, 1985; Roderick, 1996). Among the different classes and types of available molecular markers for studies of insects (Behura, 2006), sequences of mitochondrial DNA (mtDNA) have been successfully used for population genetics studies of several groups of Lepidoptera (Porreta et al., 2007), including analyzes of population structure Several studies have investigated mainly the subunits I and II of the cytochrome c oxidase gene (cox1 and cox2) (Brower, 1994a; Caterino et al., 2001; SilvaBrandão et al., 2005). Since the suitability of cox1 for species identification and delimitation was proposed (‘DNA barcode’: Hebert et al., 2004), the use of cox1 has outnumbered the use of other mitochondrial regions in studies with animals, including many Lepidoptera (Silva-Brandão et al., 2009). The Fall Armyworm, Spodoptera frugiperda (J.E. Smith) (Insecta: Lepidoptera: Noctuidae) isa devastating pest of maize that is native to tropical and subtropical regions of the western hemisphere from the United States of America to Argentina. The scientific name fallArmyworm, Spodoptera frugiperda is derived from the feeding habits of the larval life stage, frugiperda meaning “lost fruit” in Latin, as the pest can cause damage to crops resulting in severe yield loss. Severe incidences of fall armyworm were reported from across different Indian states. In India, it was first noticed in mid-May 2018 in Shivamogga, Karnataka by Sharanabasappa et al. 2018. So far, FAW is reported from Karnataka, Telangana, Andhra Pradesh, Maharashtra, Gujarat and Tamil Nadu. Because of the excessive use of insecticides to its control, FAW has already evolved resistance (McCaffery, 1998), which makes it particularly difficult to manage. However, for these individuals to be effectively considered as a source of susceptibility to resistance management, alternative hosts should contain a population able to randomly mate with individuals emerging from different crops Therefore, studies on genetic structure of insect pests are especially important in designing resistance management strategies. | ||
| Experiment Group | Basic Science | ||
| Unit Type | (02)EDUCATION UNIT | ||
| Unit | (20)ASPEE SHAKILAM BIOTECHNOLOGY INSTITUTE (SURAT) | ||
| Department | (312)ASPEE Shakilam Biotechnology Institute | ||
| BudgetHead | (339/12248/00)339/03/REG/01870 | ||
| Objective |
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| Season | Kharif | ||
| Location Unit Type | (02)EDUCATION UNIT | ||
| Location Unit | (20)ASPEE SHAKILAM BIOTECHNOLOGY INSTITUTE (SURAT) | ||
| Location Department | (312)ASPEE Shakilam Biotechnology Institute | ||
| Plot No | NA | ||
| PI Name | (NAU-EMP-2014-000487)RISHEE KISHORBHAI KALARIA | ||
| PI Email | risheekal@nau.in | ||
| PI Mobile | 9427770350 | ||
| Year of Approval | 2024 | ||
| Commencement Year | 2024 | ||
| Completion Year | 2027 | ||
| Design of Experiment |
NA |
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| Crop Spacing (cm x cm) |
NA |
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| Gross Plot (m x m) | NA | ||
| Net Plot (m x m) | NA | ||
| Total Experiment Area (m2) | NA | ||
| Plot History Last Three Year |
NA |
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| Initial Soil Sample Analysis Report |
NA |
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| Initial Soil Sample Analysis Report Attachment | Attachment Not Available! | ||
| Layout Plan |
NA |
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| Layout Plan Attachment | Attachment Not Available! | ||
| Treatment |
NA |
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| Treatment Attachment | Attachment Not Available! | ||
| (NAU-EMP-2014-000487) RISHEE KISHORBHAI KALARIA | risheekal@nau.in | 9427770350 | 29-08-2024 |
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| (NAU-EMP-2014-000483) HIREN VALLABHBHAI PATEL | hirenento30@nau.in | 9723280018 | 30/08/2024 |
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| (NAU-EMP-2014-000483) HIREN VALLABHBHAI PATEL | hirenento30@nau.in | 9723280018 | 17/05/2024 |
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| (NAU-EMP-2015-000604) NIRAV VINUBHAI RADADIYA | niravpatel@nau.in | 9879119891 | 17/05/2024 |
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| (NAU-EMP-1990-000620) BHARATKUMAR KANJIBHAI DAVDA | bharat.davda@nau.in | 9377567000 | 17/05/2024 |
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