Experiment code 20.6.3.18
Experiment Title Isolation and screening of photosynthetic microbes as single cell protein source
Research Type Lab Research
Experiment Background Crude or refined protein derived from algae, bacteria, mold, or yeast that is used for human or animal consumption is referred to as single-cell protein (SCP). Due to its high protein content, the synthesis and use of microbial biomass as a food source has drawn special attention as an alternative to proteins originating from agriculture. SCP contains lipids, vitamins, and other nutraceutical molecules in addition to proteins. The term "algae as a source of SCP" can refer to either prokaryotic cyanobacteria or small single-cell true algae, both of which grow by using light energy and carbon dioxide (autotrophic growth). Algae are frequently cultivated using methods similar to conventional agriculture, in contrast to other SCP-producing organisms, since depend on large areas and sunlight radiation. Hence, present study aims to isolate and explore their its biochemical characterization for SCP
Experiment Group Basic Science
Unit Type (02)EDUCATION UNIT
Unit (12)NAVINCHANDRA MAFATLAL COLLEGE OF AGRICULTURE (NAVSARI)
Department (223)Food Quality Testing Laboratory Navsari
BudgetHead (303/12024/00)303/01/REG/00032
Objective

To explore photosynthetic microbes as single cell protein source
Season Not season specific
Location Unit Type (02)EDUCATION UNIT
Location Unit (12)NAVINCHANDRA MAFATLAL COLLEGE OF AGRICULTURE (NAVSARI)
Location Department (223)Food Quality Testing Laboratory Navsari
Plot No Not applicable
PI Name (NAU-EMP-2013-000335)TRUPTI KANTILAL VYAS
PI Email vyastrupti@nau.in
PI Mobile 9662030836
Year of Approval 2024
Commencement Year 2025
Completion Year 2026
Design of Experiment

DIscriptive Statistics
Crop Spacing (cm x cm)

Not Applicable
Gross Plot (m x m) Not Applicable
Net Plot (m x m) Not Applicable
Total Experiment Area (m2) Not Applicable
Plot History Last Three Year

Not Applicable
Initial Soil Sample Analysis Report

Not Applicable
Initial Soil Sample Analysis Report Attachment Attachment Not Available!
Layout Plan

Not Applicable
Layout Plan Attachment Attachment Not Available!
Treatment
  • Isolation and purification of photosynthetic microbes. For isolation, fresh and marine water samples will be collected.
  • Screening for higher protein production. Spirulina (available in tablet form will be taken from market) will be kept as positive control
  • Estimation of other nutraceuticals compounds (antioxidant activity, total phenol, Flavonoid, Ascorbic Acid)
  • Identification of potential isolate by 16S rDNA/18S rDNA 

Methodology

Experiment will be carried out as described in following flow chart :

  • Samples collection - fresh and marine water
  • Purification of microbes
  • Morphological and growth characteristics of pure culture
  • Screening of isolates for higher protein content
  • Potential microbes will be identified using 16S rDNA/18S rDNA
  • Pure culture will be grown for mass production followed by drying
  • Total phenol, flavanoid and ascorbic acid content will be quantified from dry cell biomass

 

All the parameters will be monitored as per standard reference mentioned below :

  • Protein – Protein estimation will be carried out from dry cell biomass using Folin and Lowery’s method
  • Total phenol – Total phenol will be estimated from dry cell biomass using Slinkard and Singleton, 1977
  • Flavonoid  - Flavanoids from dry cell biomass will be estimated as method described by Zhishen et al., 1999
  • Ascorbic Acid – Ascorbic acid content will be estimated using Loeffler and Ponting, 1942

Identification – Potential protein containing microbes will be identified as method described by Murthy & Vyas, 2015

Observation to recorded :

  • Morphological and growth characteristics of pure culture
  • Protein content (µg/g dry weight)
  • Total phenol (µg/g dry weight)
  • Flavonoid  (µg/g dry weight)
  • Ascorbic Acid (µg/g dry weight)
  • Sequence homology
Treatment Attachment Attachment Not Available!
(NAU-EMP-2013-000335) TRUPTI KANTILAL VYAS vyastrupti@nau.in 9662030836 17-01-2025
Active
(NAU-EMP-2012-000333) SUSHEEL BRAJ MOHAN SINGH susheelsingh@nau.in 9998286581 17/01/2025
Active
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