| Experiment code | 20.6.3.17 | ||
|---|---|---|---|
| Experiment Title | Effect of PQQ against NaCl induced stress in Tomato plants at seedling stage | ||
| Research Type | Departmental Research | ||
| Experiment Background | Pyrroloquinoline-quinone (PQQ) has been implicated as a plant growth regulator in several studies. However, a detailed mode of action of the molecule is yet to be described in plants. It is known to be an antioxidant and a redox cofactor. Natural and nature identical molecules that improve the seed germination and plant growth are helpful in designing novel organic inputs. In order to assess the usability of PQQ to design such formulations, effect of various concentrations of synthetic PQQ on the seed germination and growth characteristics of tomato needs to be studied. Pyrroloquinoline quinone has been described as a plant growth regulator, incorporation of different concentrations of PQQ by plant seeds not only affected the germination rate and percentage, but also had positive effect on salt stress of plants arising from the treated seeds. A well-developed lateral root system also improves the soil area coverage by the roots thus ensuring improved nutrient uptake and better water holding capacity. This renders a plant stress resistant especially in drought prone soils. More such studies of the effect of PQQ on various plant species and at different stages of plant growth and development would help in designing the most effective method of its applications. | ||
| Experiment Group | Basic Science | ||
| Unit Type | (02)EDUCATION UNIT | ||
| Unit | (20)ASPEE SHAKILAM BIOTECHNOLOGY INSTITUTE (SURAT) | ||
| Department | (312)ASPEE Shakilam Biotechnology Institute | ||
| BudgetHead | (339/12248/00)339/03/REG/01870 | ||
| Objective |
1.To evaluate the effects of NaCl stress in tomato plants under invitro condition. 2.To explore the impact of PQQ on tomato plants under NaCl stress conditions. |
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| Season | - | ||
| Location Unit Type | (02)EDUCATION UNIT | ||
| Location Unit | (20)ASPEE SHAKILAM BIOTECHNOLOGY INSTITUTE (SURAT) | ||
| Location Department | (312)ASPEE Shakilam Biotechnology Institute | ||
| Plot No | -- | ||
| PI Name | (NAU-EMP-2012-000489)KRUNAL GOVINDBHAI MODI | ||
| PI Email | kgmodi@nau.in | ||
| PI Mobile | 9375758416 | ||
| Year of Approval | 2024 | ||
| Commencement Year | 2024 | ||
| Completion Year | 2027 | ||
| Design of Experiment |
FCRD |
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| Crop Spacing (cm x cm) |
NA |
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| Gross Plot (m x m) | - | ||
| Net Plot (m x m) | - | ||
| Total Experiment Area (m2) | - | ||
| Plot History Last Three Year |
- |
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| Initial Soil Sample Analysis Report |
- |
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| Initial Soil Sample Analysis Report Attachment | Attachment Not Available! | ||
| Layout Plan | |||
| Layout Plan Attachment | Attachment Not Available! | ||
| Treatment |
1.Tomato seeds will be germinated in basal MS media with NaCl concentration (Na) (25, 50, 100, 200, 300, 400, 500, 600 mM) to determine NaCl toxicity threshold. 2.Three NaCl concentrations (LD50, One above the LD 50 and One below the LD 50) and PQQ concentrations (0, 20 and 40 PPM) will be used to find optimal concentration of PQQ. 3.After 15 days, plants will be collected for shoot height, root length, chlorophyll content. 4.Same Plant materials will be used for enzyme assay by standard protocols. |
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| Treatment Attachment | Attachment Not Available! | ||
| (NAU-EMP-2012-000489) KRUNAL GOVINDBHAI MODI | kgmodi@nau.in | 9375758416 | 23-01-2025 |
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| Tomato | |