Experiment code 20.6.3.13
Experiment Title Exploring the protective potential of PQQ against lead-induced stress in Mung bean (Vigna radiata L.) at Seedling stage
Research Type Departmental Research
Experiment Background Bio-stimulators are a subset of bio-regulators that play a significant role in the regulation of various plant life processes. These substances come from natural sources such as plants, animals or microorganisms. Although the impact of certain regulatory molecules such as pyrroloquinoline quinone (PQQ) on animal and human functionality has been extensively studied, the functional aspects of PQQ in plant development have not been extensively researched. Despite their presence in plant species, numerous molecules with established functions in animals have yet to be fully characterized for their roles in plants. Pyrroloquinoline-quinone (PQQ), originally known as methoxatin, was first discovered as a coenzyme for methanol dehydrogenase in methylotrophic bacteria. Although PQQ is not produced by plants or animals, it is present in the tissues of animals in quantities ranging from nanograms to grams. PQQ exhibits antioxidant properties and functions as a redox cofactor. It is water-soluble and heat-stable and can facilitate redox cycles. Rhizobacteria primarily produce luconic acid or use acid phosphatase enzymes for solubilization of phosphates. Glucose dehydrogenase, a key enzyme in the biosynthesis of glucuronic acid, relies on PQQ as a cofactor for enzymatic activity A significant increase in the weight of cucumber seedlings exposed to different concentrations of synthetic PQQ indicates that this compound has a promising ability to enhance crop yield. The impact of climate change and human activities on the environment has a direct impact on the quality of the soil, which in turn affects the productivity of crops and related rhizobacterium. In view of the increasing awareness of the risks associated with chemical and inorganic substances in agriculture, bio-fertilizers, bio-pesticides and naturally occurring or identical plant growth regulators have attracted considerable attention. In order to improve crop productivity and to address the depletion of soil nutrients, sustainable agricultural methods are highlighted. Arsenic (As) is the most toxic heavy metal, followed by lead (Pb), which has no biological function. Pb toxicity has an adverse effect on plants throughout their life cycle, from seed germination to yield. A comprehensive analysis of naturally occurring non-toxic substances, such as PQQ, which promote plant growth under suboptimal conditions will facilitate the development of optimal application techniques for improving crop production and sustainability. In the present study, we investigated the protective potential of PQQ against lead-induced stress in Mung bean (Vigna radiata L.).
Experiment Group Basic Science
Unit Type (02)EDUCATION UNIT
Unit (20)ASPEE SHAKILAM BIOTECHNOLOGY INSTITUTE (SURAT)
Department (312)ASPEE Shakilam Biotechnology Institute
BudgetHead (339/12248/00)339/03/REG/01870
Objective
  1. To establish a standardized in vitro methodology to evaluate the effects of lead toxicity on mung bean.
  2. To explore the impact of PQQ on mung bean plants under lead-induced stress conditions.
Season -
Location Unit Type (02)EDUCATION UNIT
Location Unit (20)ASPEE SHAKILAM BIOTECHNOLOGY INSTITUTE (SURAT)
Location Department (312)ASPEE Shakilam Biotechnology Institute
Plot No -
PI Name (NAU-EMP-2013-000485)HARSURBHAI MANGLUBHAI JAJDA
PI Email hmjajda@nau.in
PI Mobile 8128699171
Year of Approval 2024
Commencement Year 2024
Completion Year 2026
Design of Experiment

FCRD

Crop Spacing (cm x cm)

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Gross Plot (m x m) -
Net Plot (m x m) -
Total Experiment Area (m2) -
Plot History Last Three Year

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Initial Soil Sample Analysis Report

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Initial Soil Sample Analysis Report Attachment Attachment Not Available!
Layout Plan

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Layout Plan Attachment Attachment Not Available!
Treatment
  1. Mung bean seeds will be germinated in basal MS media with lead concentrations (0,1,2,3,4,5,6,7,8,9 and 10 mM)(Lead acetate) to determine lead toxicity threshold.
  2. Five lead concentrations, including LD50, and PQQ concentrations (20 and 40 PPM) will be used to find optimal concentration of PQQ.
Treatment Attachment Attachment Not Available!
(NAU-EMP-2013-000485) HARSURBHAI MANGLUBHAI JAJDA hmjajda@nau.in 8128699171 24-01-2025
Active
(NAU-EMP-2012-000489) KRUNAL GOVINDBHAI MODI kgmodi@nau.in 9375758416 29/01/2025
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(NAU-EMP-2015-000481) HIMANSHU DHIRAJLAL BHIMANI himanshubhimani@nau.in 9426422426 29/01/2025
Active
(NAU-EMP-2008-000497) SANJAY MOHAN JHA sanjayjha14@nau.in 7600059128 29/01/2025
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