9.

Experimental detail

:

Isolate DNA from the sample followed by amplification of  the target DNA barcode region using PCR. Sequence the PCR products and Compare the resulting sequences against reference databases to find the matching species

Marker: Chloroplast rbcL, matK etc. region specific markers

Methodology:

Tissue Sampling

2. Tissue Lysis and DNA Extraction

3. Polymerase Chain Reaction – Chloroplast specific
    markers 

4. PCR Cleanup

5. Cycle Sequencing Reaction

6. Sequencing Reaction Cleanup,

7. Sequencing

8. Post Sequencing Analysis

NCBI Gene bank search for sequence and also at the International Barcode of Life (IBOL) database, BOLD (Barcode of Life Online Database); their records are not all in GenBank®. International Barcode of Life: http://www.ibolproject.org The Consortium for the Barcode of Life:   http://www.barcoding.si.edu

10.

Observation to be recorded

:

• Quantitative and Qualitative analysis of extracted genomic DNA using Electrophoratic and Nanodrop analysis.
• DNA amplification will be carried out by Termalcycler machine followed by Electrophoratic analysis.
• Gel snap will be documented under UV gel documentation system.
• Amplicone size polymorphism (bp)
• Nucleotide level polymorphism of DNA sequence

Phylogenetic analysis of species.

11.

Statement of progress

:

Total 33 species of  bamboo and seven species of Ficus have been collected. The DNA extraction process also completed for the collected samples. The DNA barcoding of 33 species of bamboo for one marker has been completed. Remaining species collection  of Ficus is in progress  and DNA barcoding work will be initiated as soon as completion of total sample collection.

Table 1: List of different Bamboo species.

Sr.

No.

Bamboo species

Local Name

1

Bambusa balcooa

Beema bamboo

2

Bambusa bambos

Katas vansh

3

Sasa auricoma

Path bamboo

4

Bambusa japonica

Garden hedge bamboo

5

Bambusa jantiana/Asamica

-

6

Bambusa multiplexa

Farm hedge bamboo

7

Bambusa nutans

-

8

Bambusa polymorpha

Burmese bamboo

9

Bambusa tulda

-

10

Bambusa vulgaris green

Green bamboo

11

Bambusa vulgaris yellow

Yellow bamboo

12

Bambusa wamin

Buddha bamboo

13

Dendrocalamus asper

Edible bamboo

14

Dendrocalamus brandisii

Velvet bamboo

15

Dendrocalamus giganteus

Giant bamboo

16

Dendrocalamus hamiltonii

-

17

Dendrocalamus longipathus

Long sheath bamboo

18

Dendrocalamus sikkimensis

-

19

Dendrocalamus stocksii

Govagiri bamboo

20

Dendrocalamus strictus

Manavel/ Bharat vansh

21

Guadua aungustifolia

Iron bamboo

22

Melocanna bambusoides

Muli bamboo

23

Sasa palmata

-

24

Schizostachym pergeacile

Pergeacile Bamboo

25

Schizostachyum dullooa

Dolloo bamboo

26

Oxytenthra parviflora

Hill bamboo

27

Thyrostachys oliveri

-

28

Ochlandra travancorica

-

29

Gigantochloa atroviolacea

Java black bamboo

30

Gigantochloa rostrata

-

Table 2: List of different Ficus species.

Name

Local Name

1

Ficus amplissima

Payar/Pipari

2

Ficus arnottiana

Khadak payar

3

Ficus asperrima

Karvati

4

Ficus benghalensis var. benghalensis

Vad

5

Ficus benghalensis var. krishnae

Krishna vad

6

Ficus benjamina

-

7

Ficus elastica

Indian rubber tree

8

Ficus hispida

Dedh umaro

9

Ficus infectoria / Ficus virens -

Pipali

10

Ficus microcarpa 

Bodhi vad

11

Ficus mysorensis

-

12

Ficus racemosa / Ficus glomerata

Audanber, Umaro

13

Ficus religiosa

Pipal