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Experiment code
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18.8.3.13 |
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Experiment Title
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Metagenomic analysis of flooded rice soil ecosystem under climate change resilience |
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Research Type
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Departmental Research |
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Experiment Background
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Methanogens and methanotrophs seem worthy of study as the organisms directly responsible for the emission of methane from soil with global emission rate from paddy fields at 60 Tg/yr. The major pathways of CH4 production in flooded soils are the reduction of CO2 with H2, with fatty acids or alcohols as hydrogen donor, and the transmethylation of acetic acid or methanol by methane-producing bacteria. The global warming potential of methane is 21 times that of carbon dioxide. Namely, compared in equimolecular terms, the greenhouse effect of methane is 21 times greater than that of carbon dioxide. As a result of these studies, some solutions are expected to be developed to reduce methanogenic activity or motivate oxidation of methane by methanotrophs to reduce the methane emission from flooded soils, especially rice fields. |
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Experiment Group
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Basic Science |
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Unit Type
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(02)EDUCATION UNIT |
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Unit
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(14)COLLEGE OF FORESTRY (ACHF-NAVSARI) |
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Department
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(279)Basic Science and Humanities,COF, Navsari |
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BudgetHead
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(329/18226/00)329/49/REG/03617 |
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Objective
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- To identify the soil microbial community by metagenomic profiling
- To compare the microbial diversity present over environment with respect to methane emission
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Season
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Not season specific |
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Location Unit Type
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(02)EDUCATION UNIT |
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Location Unit
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(14)COLLEGE OF FORESTRY (ACHF-NAVSARI) |
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Location Department
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(279)Basic Science and Humanities,COF, Navsari |
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Plot No
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NA |
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PI Name
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(NAU-EMP-2010-000958)VIPULKUMAR BHARATBHAI PATEL |
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PI Email
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vipulbiotech@nau.in |
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PI Mobile
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9825992766 |
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Year of Approval
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2022 |
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Commencement Year
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2023 |
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Completion Year
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2026 |
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Design of Experiment
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Statistical Design
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FCRD
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Experimental detail
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:
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- Sampling site: N.A.U farm.
- Site for sampling: Three site
- Sample collection time:
1. Before Transplanting (Before puddling)
2. One month after transplanting (submerged soil)
3. Two month after transplanting
Sample size: 3 Site X 3-time factor = 6
- Replications: 3
- Design: FCRD
- Molecular work: Forest Biotechnology Laboratory
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Crop Spacing (cm x cm)
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NA
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Gross Plot (m x m)
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NA |
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Net Plot (m x m)
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NA |
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Total Experiment Area (m2)
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NA |
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Plot History Last Three Year
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NA
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Initial Soil Sample Analysis Report
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NA
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Initial Soil Sample Analysis Report Attachment
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Attachment Not Available!
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Layout Plan
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NA
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Layout Plan Attachment
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Attachment Not Available! |
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Treatment
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Statistical Design
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FCRD
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Experimental detail
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:
|
- Sampling site: N.A.U farm.
- Site for sampling: Three site
- Sample collection time:
1. Before Transplanting (Before puddling)
2. One month after transplanting (submerged soil)
3. Two month after transplanting
Sample size: 3 Site X 3-time factor = 6
- Replications: 3
- Design: FCRD
- Molecular work: Forest Biotechnology Laboratory
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Observation to be recorded
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:
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- DNA quantification and quality analysis
- DNA Sequence data acquisition by 16s r DNA / NGS platform (As per available resources )
- Data analysis on Bioinformatics platforms
- SOM
- Available Nutrients: N, P, K, S, Fe and Zn
- Weather parameters: Temperature, humidity, sunshine hours etc.
- Methane flux at the time of soil sampling
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Treatment Attachment
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Attachment Not Available! |