Experiment code 20.3.3.16
Experiment Title Dissipation behaviour and dietary risk assessment of combi-product Chlorantraniliprole and λ-cyhalothrin residues in/on Indian bean (Lablab purpureus L.)
Research Type Departmental Research
Experiment Background Indian beans (Lablab purpureus L.; Family Fabaceae) are popular vegetable in India is a poor man’s meat as it has many nutritional benefits and it is the best source of digestible vegetable protein (20-25%) required for human health.It is multipurpose crop use as vegetable, pulse and forage. A wide range of pesticides are used for the production of Indian beans. Nowadays, farmers across the globe preferred amalgam of pesticides over its single counterparts due to a broad spectrum of pest control with cost-effectiveness and resistance management in pests. The mixture of chlorantraniliprole 9.26 % and λ-cyhalothrin 4.63 % ZC has been registered in India to cater the protection from pod borer problem in pigeonpea (CIBRC, 2023).Insecticides viz., Flubendiamide, Indoxacarb, λ-cyhalothrin, and Chlorantraniliprole etc. are extensively used to control the pod borer infestation problem in Indian bean. These pesticides have potentially adverse effect on human health. Especially, South Gujarat is major producer of Indian bean. Therefore, it becomes essential to study the behaviour and dietary risk assessment of combi-product Chlorantraniliprole and λ-cyhalothrin residues in/on Indian bean.
Experiment Group Plant Protection
Unit Type (02)EDUCATION UNIT
Unit (12)NAVINCHANDRA MAFATLAL COLLEGE OF AGRICULTURE (NAVSARI)
Department (223)Food Quality Testing Laboratory Navsari
BudgetHead (303/12024/00)303/01/REG/00032
Objective

 

  • To study the dissipation behaviour of Chlorantraniliprole and λ-cyhalothrinresidues in/on Indian bean
  • To work out the dietary risk assessment of  Chlorantraniliprole and λ-cyhalothrin for Indian bean
Season Late-Kharif
Location Unit Type (02)EDUCATION UNIT
Location Unit (12)NAVINCHANDRA MAFATLAL COLLEGE OF AGRICULTURE (NAVSARI)
Location Department (223)Food Quality Testing Laboratory Navsari
Plot No Not Applicable
PI Name (NAU-EMP-2012-000333)SUSHEEL BRAJ MOHAN SINGH
PI Email susheelsingh@nau.in
PI Mobile 9998286581
Year of Approval 2024
Commencement Year 2024
Completion Year 2026
Design of Experiment

Large plot

Crop Spacing (cm x cm)

standard

Gross Plot (m x m) -
Net Plot (m x m) -
Total Experiment Area (m2) -
Plot History Last Three Year

Not Available

Initial Soil Sample Analysis Report

Not Applicable

Initial Soil Sample Analysis Report Attachment Attachment Not Available!
Layout Plan

NOt applicable

Layout Plan Attachment Attachment Not Available!
Treatment

1.         Control – water spray

2.         Recommended dose  @ 30ga.i/ha

3.         Intermediate Dose @ 37.5 g ai/ha

4.         Double to recommended dose @ 60 ga.i/ ha

Note : Any commercial formulation of chlorantraniliprole 9.26 % and λ-cyhalothrin 4.63 % ZC will be sprayed at twice; 1st at 50 % flowering stage and followed by second spray at 15 days interval

Methodology :

Any commercial formulation of chlorantraniliprole 9.26 % and λ-cyhalothrin 4.63 % ZC will be sprayed twice; 1st at 50 % flowering stage and followed by second spray at 15 days interval at three doses specified above.

Dissipation and persistence

Sampling :

Indian bean : -0 day (2 hrs after last spray), 1, 3, 5, 7, 10, 20, 30 days

Soil : 0 day and 30 DAA

Sample collection : Indian bean pods: Approx. 500 g Indian bean pod samples will be collected from each treatment and will be subjected to residue analysis with QuEChERS approach and quantification.

Soil : Approximately 1 kg soil samples will be collected from 5 sampling sites per treatment with standard sampling procedure and processed with QuECHERS approach.

Dietary risk assessment

The residues of  chlorantraniliprole and λ-cyhalothrin in/on Indian bean obtained at 0 day were considered for dietary risk assessment calculations. The dietary exposure and risk assessment will be worked out  by using estimated daily intake (EDI) and risk quotient (RQ).

EDI = [Residue (mg/kg ) X Food consumption (kg/day )] / Body weight (kg)

Body weight and food intake for various age groups of the Indian population will be taken into account when doing the calculations in accordance with the references of the National Institute of Nutrition, Hyderabad (ICMR, 2011).

RQ = EDI / ADI

Where RQ is the risk quotient, EDI is the estimated daily intake (mg/kg , bw) and ADI is the acceptable daily intake (mg/kg , bw).

In terms of numbers, a chemical that has an RQ value greater than 1 is risky for consumers (Mariappan and Kaithamalai, 2020; Kansara et al., 2021; Singh et al., 2023).

Observations:

  • Method standardization: Linearity, Limit of detection (LOD), Limit of Quantification (LOQ) and Recovery (%)
  • Dissipation study of Chlorantraniliprole and λ-cyhalothrin (DT50, Waiting period) in Indian bean
  • Dissipation study of Chlorantraniliprole and λ-cyhalothrin (DT50) in soil
  • Chlorantraniliprole and λ-cyhalothrin residues in/on Indian bean 
  • Dietary risk assessment of Chlorantraniliprole and λ-cyhalothrin in Indian bean
Treatment Attachment Attachment Not Available!
(NAU-EMP-2012-000333) SUSHEEL BRAJ MOHAN SINGH susheelsingh@nau.in 9998286581 02-02-2025
Active
(NAU-EMP-2008-000838) DIGVIJAYSINH AMRUTSINH CHAUHAN dachauhan@nau.in 9426859819 17/05/2024
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