| Experiment code | 20.6.3.21 | ||
|---|---|---|---|
| Experiment Title | In vitro regeneration studies in Tuberose (Polianthus tuberosa L.) | ||
| Research Type | Departmental Research | ||
| Experiment Background | Tuberose (Polianthes tuberosa L.) is a member of the family Amaryllidaceae. It is one of the important bulbous flower crops of tropical and subtropical regions. Among the commercially grown flowers in India, tuberose occupies a prime position owing to its popularity as a cut flower, loose flower, for perfumery as well as its potential as source of secondary metabolites. Tuberose is commercially grown for its fragrant flowers for perfume industry in India and France. The fragrant oil, almost all of which is exported from India, is one of the most expensive of the fragrant oils used in perfumes, Tuberose is conventionally propagated by means of bulbs which are produced by the mother plant. However, this method is very slow with low multiplication i.e .one bulb can proliferate only 1.6 per annum (approximate). Conventional propagation methods have a plethora of bottlenecks such as difficulty in producing true-to-type bulbs as well as spread of diseases from stock plants infected with bacteria, fungi, mycoplasma, nematodes, viruses, etc. Thus, in vitro propagation technique proves a highly potent tool with several benefits like rapid mass multiplication of new cultivars, ease in maintenance of disease-free stock, year round production of plantlets and storage of plantlets in a small space. Tuberose does not have enough background for regeneration through tissue culture and in vitro propagation techniques in this crop have been in vogue. (Kadam et al., 2010). Keeping the above considerations in view an investigation was taken up to standardise a viable micropropagation protocol for the commercially important tuberose . | ||
| Experiment Group | Basic Science | ||
| Unit Type | (02)EDUCATION UNIT | ||
| Unit | (13)ASPEE COLLEGE OF HORTICULTURE (NAVSARI) | ||
| Department | (219)Basic and Allied Sciences,ACHF, Navsari | ||
| BudgetHead | (329/12014/05)329/07/REG/02130 | ||
| Objective |
To generate a suitable regeneration protocol for mass multiplication of tuberose |
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| Season | Not season specific | ||
| Location Unit Type | (02)EDUCATION UNIT | ||
| Location Unit | (13)ASPEE COLLEGE OF HORTICULTURE (NAVSARI) | ||
| Location Department | (219)Basic and Allied Sciences,ACHF, Navsari | ||
| Plot No | NA | ||
| PI Name | (NAU-EMP-2016-000927)REHANA - NIYARIA | ||
| PI Email | drrehana04@nau.in | ||
| PI Mobile | 9898940253 | ||
| Year of Approval | 2024 | ||
| Commencement Year | 2024 | ||
| Completion Year | 2027 | ||
| Design of Experiment |
CRD (3 repetitions) |
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| Crop Spacing (cm x cm) |
NA |
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| Gross Plot (m x m) | NA | ||
| Net Plot (m x m) | NA | ||
| Total Experiment Area (m2) | NA | ||
| Plot History Last Three Year |
NA |
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| Initial Soil Sample Analysis Report |
NA |
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| Initial Soil Sample Analysis Report Attachment | Attachment Not Available! | ||
| Layout Plan |
NA |
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| Layout Plan Attachment | Attachment Not Available! | ||
| Treatment |
For expalnt establishment: T1: MS T2: MS+0.5 mg/l BAP T3 : MS+ 1 mg/l BAP T4 : MS+ 1.5 mg/l BAP T5 : MS+ 2 mg/l BAP For shoot multiplication: T1: MS+2 mg/l BAP T2: MS+ 2.5 mg/l BAP T3 : MS+ 3.0 mg/l BAP T4 : MS+ 3.5 mg/l BAP T5 : MS+ 4 mg/l BAP For elongation: T1: MS T2: MS+2 mg/l BAP T3 : MS+ 2.5 mg/l BAP T4 : MS+ 3.0 mg/l BAP T5 : MS+ 3.5 mg/l BAP For rooting: T1: ½ MS+ 0.5 IBA T2: ½ MS+ 1.0 IBA T3 : ½ MS+ 1.5 IBA T4 : ½ MS+ 2.0 IBA T5 : ½ MS+ 2.5 IBA |
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| Treatment Attachment | Attachment Not Available! | ||
| (NAU-EMP-2016-000927) REHANA - NIYARIA | drrehana04@nau.in | 9898940253 | 05-02-2025 |
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| (NAU-EMP-2011-000975) KIRANKUMAR PARSHOTTAMBHAI SUTHAR | kiransuthar@nau.in | 9978888938 | 05/02/2025 |
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| (NAU-EMP-2008-000951) SHIVLAL GANESHLAL CHAWLA | shivlalchawla@nau.in | 9998002589 | 05/02/2025 |
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