Experiment code 20.6.3.27
Experiment Title Transcriptomics based molecular dissection of photoperiod responsive flowering in Indian bean (Lablab purpureus L. Sweet)
Research Type Departmental Research
Experiment Background Indian bean [Lablab purpureus (L.) Sweet] is a very important short day pulse crop of South Gujarat especially the tribal area. The green pods along with green seeds of this crop are used as vegetable while dried seeds are used as split pulse. Though wide variability is present in South Gujarat region for this crop, it is not extensively studied. Most of the land races and cultivars of Indian bean are photoperiod sensitive and indeterminate which flower only during short day length of winter season making its cultivation difficult across the year. Photoperiod sensitive flowering also poses difficulties in germplasm conversion when breeder is interested to transfer valuable traits from sensitive types to insensitive ones. However, few determinate and photoperiod insensitive types are available which flowers across the year. Due to synchronous flowering and maturity, determinate growth habit and photoperiod insensitive flowering enables solitary cultivation as well as inter cropping especially in plantation crops almost throughout the year. Growth habit and photoperiod responsive flowering have played very important role in evolution and domestication of Indian bean, however, the actual genes and underlying mechanism have not been identified. Knowledge on molecular pathways governing them is completely absent in literature for Indian bean. Currently, transcriptome sequencing technology is widely used in the discovery of new genes and differential expression analysis to dissect complex molecular pathways. The present study aims to identify genes involved in photoperiod responsive flowering, which will provide a deep understanding of underlying molecular mechanisms. The findings of present study will provide an important reference for the molecular breeding of other pulse crops in relation to photoperiod responsive flowering.
Experiment Group Basic Science
Unit Type (02)EDUCATION UNIT
Unit (12)NAVINCHANDRA MAFATLAL COLLEGE OF AGRICULTURE (NAVSARI)
Department (257)Genetics and Plant Breeding Department, NMCA, Navsari
BudgetHead (303/12077/00)303/01/REG/04502
Objective

1. To study differentially expressed genes under different photoperiods.

2. To validate key differentially expressed genes

Season Not season specific
Location Unit Type (02)EDUCATION UNIT
Location Unit (12)NAVINCHANDRA MAFATLAL COLLEGE OF AGRICULTURE (NAVSARI)
Location Department (257)Genetics and Plant Breeding Department, NMCA, Navsari
Plot No NA
PI Name (NAU-EMP-2011-000348)KAUSHALKUMAR GUNVANTRAY MODHA
PI Email kgmodha@nau.in
PI Mobile 9909694684
Year of Approval 2024
Commencement Year 2024
Completion Year 2026
Design of Experiment

Descriptive Statistics

Crop Spacing (cm x cm)

Not applicable

Gross Plot (m x m) NA
Net Plot (m x m) NA
Total Experiment Area (m2) NA
Plot History Last Three Year

NA

Initial Soil Sample Analysis Report

NA

Initial Soil Sample Analysis Report Attachment Attachment Not Available!
Layout Plan

NA

Layout Plan Attachment Attachment Not Available!
Treatment

1. T1: Long Day

2. T2: Short Day

Trifoliate leaves of determinate/photo-insensitive variety GNIB-21 and indeterminate / photosensitive variety GNIB-22

Treatment Attachment Attachment Not Available!
(NAU-EMP-2011-000348) KAUSHALKUMAR GUNVANTRAY MODHA kgmodha@nau.in 9909694684 11-02-2025
Active
(NAU-EMP-2010-000958) VIPULKUMAR BHARATBHAI PATEL vipulbiotech@nau.in 9825992766 01/04/2024
Active
(NAU-EMP-2012-000381) RITESHKUMAR KANUBHAI PATEL riteshpatel@nau.in 9913744025 01/04/2024
Active
(NAU-EMP-2008-000838) DIGVIJAYSINH AMRUTSINH CHAUHAN dachauhan@nau.in 9426859819 01/04/2024
Active
Indian bean GNIB 21
Indian bean GNIB-22