| Experiment code | 17.5.3.27 | ||
|---|---|---|---|
| Experiment Title | Development of tissue culture protocol for chrysanthemum novel mutants | ||
| Research Type | Departmental Research | ||
| Experiment Background | Chrysanthemum is an important ornamental crop for cut flower and pot plant production. Chrysanthemum is popularly known as Queen of the East and Autumn Queen. Chrysanthemum was named using two Greek prefixes, 'Chrys', which means golden (the colour of the original flowers), and 'Anthemon', meaning flower. Chrysanthemum is one of the important cut flowers in the international market and ranks 2nd in the global cut flower trade after rose. Chrysanthemum stands 5th in world pot plant trade after Kalanchoe, Hedera, Ficus and Saintpaulia. In Japan and China it is the number one flower. The commercial cultivars are usually propagated vegetatively through terminal cuttings and root suckers which are very slow, time consuming and tiring. These conventional propagation methods not only take longer time to flower, but also not useful for large scale production. Regeneration through in vitro culture has become now a viable alternative to the conventional propagation methods. Chrysanthemums are susceptible to infection by many viruses. Problems of virus infection can also be solved by micropropagation methods. In recent years various reports on in vitro propagation of different plant species have shown that tissue culture technique is suitable for rapid propagation of selected plant species. A number of studies have been carried out on in vitro propagation of C. morifolium. Many researchers developed micropropagation techniques for Chrysanthemum using different parts such as shoot tip, leaf, stem, apical meristem and few report from the ray florets of chrysanthemum. Present study is designed using ray florets explants which may be fruitful to cultivate the plant in our soil and environment. Most of reports regarding in vitro regeneration of Chrysanthemums from different explants via organogenesis somatic embryogenesis and callus culture. Tissue culture is alternatively called cell, tissue and organ culture through in vitro condition. It can be employed for large scale propagation of disease free clones and gene pool conservation. The present study aims at developing a simple, rapid and high frequency regeneration protocol of Chrysanthemum so as to give rise to true-to-type clones for potential application in large scale propagation. | ||
| Experiment Group | Basic Science | ||
| Unit Type | (02)EDUCATION UNIT | ||
| Unit | (12)NAVINCHANDRA MAFATLAL COLLEGE OF AGRICULTURE (NAVSARI) | ||
| Department | (353)navinchandra mafatlal college of agriculture | ||
| BudgetHead | (303/12135/00)303/03/REG/01776 | ||
| Objective |
1. Standardization of explants surface sterilization treatment of chrysanthemum 2. Standardization of media for callus induction and regeneration of chrysanthemum 3. Standardization of media for shoot proliferation of chrysanthemum 4. Standardization of media for rooting of chrysanthemum 5. Hardening of rooted micro shoots of chrysanthemum |
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| Season | Not season specific | ||
| Location Unit Type | (02)EDUCATION UNIT | ||
| Location Unit | (12)NAVINCHANDRA MAFATLAL COLLEGE OF AGRICULTURE (NAVSARI) | ||
| Location Department | (353)navinchandra mafatlal college of agriculture | ||
| Plot No | Lab Experiment | ||
| PI Name | (NAU-EMP-2008-000426)AJAY VITTHALRAO NARWADE | ||
| PI Email | narwadeajay_2000@nau.in | ||
| PI Mobile | 9375189469 | ||
| Year of Approval | 2021 | ||
| Commencement Year | 2021 | ||
| Completion Year | 2024 | ||
| Design of Experiment |
CRD |
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| Crop Spacing (cm x cm) |
NA |
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| Gross Plot (m x m) | NA | ||
| Net Plot (m x m) | NA | ||
| Total Experiment Area (m2) | NA | ||
| Plot History Last Three Year |
NA |
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| Initial Soil Sample Analysis Report |
NA |
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| Initial Soil Sample Analysis Report Attachment | Attachment Not Available! | ||
| Layout Plan |
NA |
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| Layout Plan Attachment | Attachment Not Available! | ||
| Treatment |
1) Callus induction and regeneration Callus induction: T0 = MS (Control) T1 = MS + BAP (3 mg/l) + NAA (1 mg/l) T2 = MS + BAP (3 mg/l) + NAA (2 mg/l) T3 = MS + BAP (3 mg/l) + NAA (3 mg/l) T4 = MS + BAP (4 mg/l) + NAA (1 mg/l) T5 = MS + BAP (4 mg/l) + NAA (2 mg/l) T6 = MS + BAP (4 mg/l) + NAA (3 mg/l) T7 = MS + BAP (5 mg/l) + NAA (1 mg/l) T8 = MS + BAP (5 mg/l) + NAA (2 mg/l) T9 = MS + BAP (5 mg/l) + NAA (3 mg/l) Callus regeneration T0 = MS (Control) T1 = MS + Kinetin (5 mg/l) + NAA (1 mg/l) T2 = MS + Kinetin (5 mg/l) + NAA (2 mg/l) T3 = MS + Kinetin (5 mg/l) + NAA (3 mg/l) T4 = MS + Kinetin (10 mg/l) + NAA (1 mg/l) T5 = MS + Kinetin (10 mg/l) + NAA (2 mg/l) T6 = MS + Kinetin (10 mg/l) + NAA (3 mg/l) T7 = MS + Kinetin (15 mg/l) + NAA (1 mg/l) T8 = MS + Kinetin (15 mg/l) + NAA (2 mg/l) T9 = MS + Kinetin (15 mg/l) + NAA (3 mg/l) 2) Shoot proliferation and rooting Shoot regeneration T0 = MS (Control) T1 = MS + BAP (3 mg/l) + NAA (0.1 mg/l) T2 = MS + BAP (3 mg/l) + NAA (0.5 mg/l) T3 = MS + BAP (3 mg/l) + NAA (1.0 mg/l) T4 = MS + BAP (4 mg/l) + NAA (0.1 mg/l) T5 = MS + BAP (4 mg/l) + NAA (0.5 mg/l) T6 = MS + BAP (4 mg/l) + NAA (1.0 mg/l) T7 = MS + BAP (5 mg/l) + NAA (0.1 mg/l) T8 = MS + BAP (5 mg/l) + NAA (0.5 mg/l) T9 = MS + BAP (5 mg/l) + NAA (1.0 mg/l) Shoot multiplication T0 = MS (Control) T1 = MS + BAP (3 mg/l) + NAA (0.01 mg/l) + GA3¬ (0.2 mg/l) T2 = MS + BAP (3 mg/l) + NAA (0.05 mg/l) + GA3¬ (0.2 mg/l) T3 = MS + BAP (3 mg/l) + NAA (0.1 mg/l) + GA3¬ (0.2 mg/l) T4 = MS + BAP (5 mg/l) + NAA (0.01 mg/l) + GA3¬ (0.2 mg/l) T5 = MS + BAP (5 mg/l) + NAA (0.05 mg/l) + GA3¬ (0.2 mg/l) T6 = MS + BAP (5 mg/l) + NAA (0.1 mg/l) + GA3¬ (0.2 mg/l) T7 = MS + BAP (7.5 mg/l) + NAA (0.01 mg/l) + GA3¬ (0.2 mg/l) T8 = MS + BAP (7.5 mg/l) + NAA (0.05 mg/l) + GA3¬ (0.2 mg/l) T9 = MS + BAP (7.5 mg/l) + NAA (0.1 mg/l) + GA3¬ (0.2 mg/l) Rooting T0 = ½ MS (Control) T1 = ½ MS + NAA (0.25 mg/l) T2 = ½ MS + NAA (0.50 mg/l) T3 = ½ MS + NAA (1.0 mg/l) 3) Hardening (primary and secondary hardening) Primary hardening T1 = Coco peat T2 = Vermiculite T3 = Perlite Second hardening T1 = Clay + Sand T2 = Clay + Sand + Coco peat T3 = Clay + Sand + Vermicompost |
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| Treatment Attachment | Attachment Not Available! | ||
| (NAU-EMP-2008-000426) AJAY VITTHALRAO NARWADE | narwadeajay_2000@nau.in | 9375189469 | 15-02-2025 |
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| (NAU-EMP-2008-000951) SHIVLAL GANESHLAL CHAWLA | shivlalchawla@nau.in | 9998002589 | 15/02/2021 |
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| Chrysanthemum | |