Influence of pre-sowing treatments on seed germination and early growth in seedlings of Patla [Stereospermum chelonoides (L.f.) DC.]
Research Type
Departmental Research
Experiment Background
Stereospermum chelonoides (L.f.) DC. (Syn. Stereospermum suaveolens (Roxb.) Benth & Hook. F., Family- Bignoniaceae) is commonly called as ‘Padiala or Paral’ (Hindi), ‘Patala’ (Sanskrit) and Patla (Gujarati). It is a large sized indigenous tree and distributed in sub-Himalayan tract, central parts of India and also sparsely distributed in Gujarat. Different parts of the tree are used for pharmacological purposes where root is used for hypoglycaemic and anticancer activity; stem bark for diuretic effect, antihyperglycemic, antioxidant, hepatoprotective and free radical scavenging activity.
Destructive methods of tree harvesting seriously reduced population size and it also affected on seed yield and natural regeneration. Moreover, it is propagated naturally by seeds and collection of seeds is difficult as winged pericarps dispersed by wind after mature. Further, poor germination rate affected the natural regeneration. S. chelonoides is a lesser known tree species and listed as Critically Endangered in Gujarat (Anon., 2008). Further seed germination is very meagre (Approx. 6 %) in normal condition. Hence, there is great scope for scientific exploration of the LKT species including germination attributes; further, there is a need to understand the germination characters of this species. Therefore, pre-sowing treatments for enhancement of seed germination and early seedling growth of S. chelonoides in the nursery stage is proposed.
Null Hypothesis:
There is no influence of pre-sowing treatments on seed germination and early seedling growth in Patla (Stereospermum chelonoides)
Experiment Group
Forestry
Unit Type
(02)EDUCATION UNIT
Unit
(61)COLLEGE OF FORESTRY (NAU, NAVSARI)
Department
(2360)SILVICULTURE AND AGROFORESTRY, COF, NAU, NAVSARI
BudgetHead
(352/12065/00)352/01/REG/04200
Objective
To study the influence of pre-sowing treatments on seed germination and early seedling growth in Patla(Stereospermum chelonoides)
Season
Not season specific
Location Unit Type
(02)EDUCATION UNIT
Location Unit
(61)COLLEGE OF FORESTRY (NAU, NAVSARI)
Location Department
(2360)SILVICULTURE AND AGROFORESTRY, COF, NAU, NAVSARI
Plot No
CoF Nursery
PI Name
(NAU-EMP-2010-000895)LAXMIKANTA . BEHERA
PI Email
lkbehera@nau.in
PI Mobile
9375282020
Year of Approval
2025
Commencement Year
2025
Completion Year
2028
Design of Experiment
CRD
Crop Spacing (cm x cm)
NA
Gross Plot (m x m)
Nursery experiment
Net Plot (m x m)
Nursery experiment
Total Experiment Area (m2)
Nursery experiment
Plot History Last Three Year
NA
Initial Soil Sample Analysis Report
NA
Initial Soil Sample Analysis Report Attachment
Attachment Not Available!
Layout Plan
For germination trial
For germination experiment, total fourteen different pre-sowing treatments with three replication in CRD
Layout Plan Attachment
Attachment Not Available!
Treatment
For germination trial
For germination experiment, total fourteen different pre-sowing treatments will be adopted and details are as follows:
T1- Control
T2- Soaking seeds in normal water for 24 hrs
T3- Soaking seeds in normal water for 48 hrs
T4- Soaking seeds in luke warm water for 24 hrs
T5: T2 + Soaking seeds in GA3 solution @ 50 ppm for 30 min
T6: T2 + Soaking seeds in GA3 solution @ 100 ppm for 30 min
T7: T2 + Soaking seeds in GA3 solution @ 150 ppm for 30 min
T8: T2 + Soaking seeds in GA3 solution @ 200 ppm for 30 min
T9: T2 + Soaking seeds in GA3 solution @ 50 ppm for 60 min
T10: T2 + Soaking seeds in GA3 solution @ 100 ppm for 60 min
T11: T2 + Soaking seeds in GA3 solution @ 150 ppm for 60 min
T12: T2 + Soaking seeds in GA3 solution @ 200 ppm for 60 min
T13- Soaking seeds in cow dung slurry for 24 hrs
T14- Soaking seeds in cow dung slurry for 48 hrs
CRD with three replication
In first experiment of germination, for each treatment, 3 repetitions will be used and for each repetition, 50 seeds will be considered (N= 2100 seeds for whole experiment). Seeds will be sown on germination tray containing sand media. Daily germination count will be recorded up to 30 days. For the second experiment of growth, best 5 treatments out of 14 treatments will be considered for the study. Two leaf stage seedlings from five treatments will be transplanted into polybags of 6² x 8² size containing Soil+ Sand + FYM (2:1:1) for growth study. For each treatment, 60 seedlings (20 seedlings per repetition) will be employed for growth and biomass observations.
Observations to be recorded:
Germination data
Germination percentage
Mean Daily Germination (MDG)
Peak Value of Germination (PV)
Germination Value (GV)
Mean Germination Time (MGT)
Germination Rate Index (GRI)
Seedling growth and vigour parameters
Shoot height (cm)
Collar diameter (cm)
Number of leaves per plant
Fresh weight (shoot and root separately) of plant (g)
Dry weight (shoot and root separately) of plant (g)
Root: Shoot ratio
Sturdiness quotient
Seedling quality index
Survivability (%)
Seedling growth and vigour parameters should be taken after 6 months of transplanting