Experiment code Agro-13
Experiment Title Bio-chemical changes in leafy vegetables grown on contaminated and Non-contaminated soils
Research Type Departmental Research
Experiment Background Many researchers and scientists from India and abroad, after studying soil (Kumar and Srikantaswamy, 2012; Jayashreeet al. 2012; Dheeba B. et al. 2012), water (Lokhandeet al., 2011; Kumar et al. 2011) and plant samples (Sharma et al. 2009; Tanushreeet al. 2011) from industrial and adjacent areas, reported varying degrees of contamination of heavy metals in soil-water-plant systems which ultimately were polluting not only aquatic resources and lives but also agricultural and other plants/crops which later on became a part of food chain and thereby becoming harmful to human and animal kingdom. Together with rapid worldwide industrialization, heavy metals have become one of the major types of environmental contaminants, particularly in soils (Qin et al., 2012). These elements can be phytotoxic and harmful to vital microbial processes in nutrient cycling (Oliveira et al., 2005). Chemical analyses provide information only referring to the accumulation of these contaminants in soils, but they will not indicate the consequences they have on soil biochemical composition and metabolism. Keeping all the above points in view, a pot experiment has been planned on the evaluation of bio-chemical properties of different leafy vegetables grown on contaminated and non-contaminated soil.
Experiment Group Natural Resource Management
Unit Type (02)EDUCATION UNIT
Unit (21)COLLEGE OF AGRICULTURE (BHARUCH)
Department (328)Soil Science
BudgetHead (337/12246/00)337/03/REG/01861
Objective
  1. To assess biochemical changes in different leafy vegetables grown in contaminated and non-contaminated soils
  2. To assess the accumulation of heavy metals in leafy vegetables
  3. To evaluate nutrient content of leafy vegetables grown in contaminated and non-contaminated soils
  4. Phytoaccumulation of heavy metals and Bio-concentration Factor (BCF) of shoot and root in the leafy vegetables grown in contaminated and non-contaminated soils
Season Annual
Location Unit Type (02)EDUCATION UNIT
Location Unit (21)COLLEGE OF AGRICULTURE (BHARUCH)
Location Department (328)Soil Science
Plot No Green House
PI Name (NAU-EMP-2019-000681)SURESHKUMAR MAGANBHAI BAMBHANEEYA
PI Email sureshsoilscience@nau.in
PI Mobile 7874206663
Year of Approval 2020
Commencement Year 2020
Completion Year 2022
Design of Experiment

Plot No. : - Under net house                                                                N                          

 

S1V1

 

S1V2

 

S1V3

 

S1V4

 

S1V5

 

 

 

 

 

 

 

 

 

S1V1

 

S1V2

 

S1V3

 

S1V4

 

S1V5

 

 

 

 

 

 

 

 

 

S1V1

 

S1V2

 

S1V3

 

S1V4

 

S1V5

 

S2V1

 

S2V2

 

S2V3

 

S2V4

 

S2V5

 

 

 

 

 

 

 

 

 

S2V1

 

S2V2

 

S2V3

 

S2V4

 

S2V5

 

 

 

 

 

 

 

 

 

S2V1

 

S2V2

 

S2V3

 

S2V4

 

S2V5

 

 

 

Crop Spacing (cm x cm)

30 x 30 cm

Gross Plot (m x m) Pot experiment: Pot dimension- 30 x 30 cm
Net Plot (m x m) Pot experiment: Pot dimension- 30 x 30 cm
Total Experiment Area (m2) Pot experiment
Plot History Last Three Year
  1. First year soil survey will be conducted from leafy vegetables growing around Ankleshwar and Bharuch region. identify various locations of both the areas for digging out representative soil samples (0-15 cm) using GPS system. Based on preliminary survey and other information of surface samples (total 50 nos.) will be done from the above areas.
  2. Soil chemical analysis will be done and afterwards contaminated and non-contaminated soils on the basis of maximum permissible limits of heavy metal concentration as given by WHO(1996).
  3. Pot culture trial will be conducted for soil and plant samples analysis at College.
Initial Soil Sample Analysis Report

Table:-  2a. Soil chemical properties in initial soil samples collected from contaminated area.

Sample ID

pH

EC

(dS m-1)

SOC

(%)

N

P2O5

K2O

Fe

Zn

(kg ha-1)

 

A1

7.85

0.98

0.42

219.5

20.5

403.7

4.62

0.28

A2

8.98

1.20

0.38

156.8

11.2

337.9

3.70

1.97

A3

9.84

0.48

0.14

125.4

39.2

285.5

0.78

10.98

A4

7.85

0.58

0.24

250.9

15.4

297.6

3.18

0.26

A5

6.89

0.36

0.27

131.4

15.6

423.4

7.78

6.51

Min.

6.89

0.36

0.14

125.4

11.2

285.5

0.78

0.26

Max.

9.84

1.20

0.42

250.9

39.2

423.4

7.78

10.98

Mean

8.28

0.72

0.29

176.8

20.4

349.6

4.01

4.00

B1

6.51

0.58

0.38

188.2

12.1

297.6

7.08

1.39

B2

8.45

1.25

0.50

156.8

14.9

315.2

6.90

0.97

B3

7.26

1.39

0.18

125.4

12.2

330.4

9.38

1.92

B4

8.91

0.84

0.38

94.1

33.6

320.4

11.30

0.29

B5

6.12

2.64

0.24

131.4

17.5

275.4

10.90

0.22

Min.

6.12

0.58

0.18

94.1

12.1

275.4

6.90

0.22

Max.

8.91

2.64

0.50

188.2

33.6

330.4

11.30

1.92

Mean

7.45

1.34

0.34

139.2

18.1

307.8

9.11

0.96

C1

7.95

0.47

0.20

407.7

21.5

261.3

2.04

0.27

C2

6.31

1.10

0.14

658.6

48.5

163.3

5.00

0.03

C3

8.45

1.60

0.12

219.5

89.6

181.6

10.40

0.03

C4

9.45

1.20

0.20

194.1

56.0

275.5

7.08

0.24

C5

9.84

0.89

0.24

131.4

15.6

422.0

12.10

0.24

Min.

6.31

0.47

0.12

131.4

15.6

163.3

2.04

0.03

Max.

9.84

1.60

0.24

658.6

89.6

422.0

12.10

0.27

Mean

8.40

1.05

0.18

322.3

46.2

260.7

7.32

0.16

D1

6.58

0.69

0.19

125.4

11.9

294.9

2.18

2.54

D2

8.41

0.48

0.20

156.8

76.6

409.2

5.16

3.17

D3

7.25

1.25

0.21

313.6

70.7

324.6

2.02

4.28

D4

6.01

1.14

0.35

407.7

60.6

310.5

2.72

4.72

D5

6.90

1.05

0.14

345.0

57.5

454.8

1.96

1.96

Min.

6.01

0.48

0.14

125.4

11.9

294.9

1.96

1.96

Max.

8.41

1.25

0.35

407.7

76.6

454.8

5.16

4.72

Mean

7.03

0.92

0.22

269.7

55.5

358.8

2.81

3.33

E1

7.12

1.25

0.18

156.8

33.2

448.1

1.26

0.33

E2

8.45

1.02

0.63

154.8

56.0

310.5

0.42

0.92

E3

6.58

0.48

0.59

188.2

30.2

262.6

5.38

2.05

E4

8.45

0.69

0.38

439.0

18.4

356.7

0.92

0.27

E5

9.25

0.89

0.17

376.3

33.6

323.9

2.50

0.99

Min.

6.58

0.48

0.17

154.8

18.4

262.6

0.42

0.27

Max.

9.25

1.25

0.63

439.0

56.0

448.1

5.38

2.05

Mean

7.97

0.87

0.39

263.0

34.3

340.4

2.10

0.91

F1

7.36

0.65

0.29

250.9

19.2

230.4

3.00

0.27

F2

8.45

0.66

0.24

313.6

48.5

248.6

3.20

0.64

F3

9.45

1.01

0.17

125.4

44.8

260.7

1.88

0.50

F4

6.14

1.05

0.20

101.4

56.0

270.1

1.70

0.65

F5

8.40

1.10

0.80

85.6

65.2

276.9

1.88

0.43

Min.

6.14

0.65

0.17

85.6

19.2

230.4

1.70

0.27

Max.

9.45

1.10

0.80

313.6

65.2

276.9

3.20

0.65

Mean

7.96

0.89

0.34

175.4

46.7

257.3

2.33

0.50

Initial Soil Sample Analysis Report Attachment
Layout Plan

S1V1

 

S1V2

 

S1V3

 

S1V4

 

S1V5

 

 

 

 

 

 

 

 

 

S1V1

 

S1V2

 

S1V3

 

S1V4

 

S1V5

 

 

 

 

 

 

 

 

 

S1V1

 

S1V2

 

S1V3

 

S1V4

 

S1V5

 

S2V1

 

S2V2

 

S2V3

 

S2V4

 

S2V5

 

 

 

 

 

 

 

 

 

S2V1

 

S2V2

 

S2V3

 

S2V4

 

S2V5

 

 

 

 

 

 

 

 

 

S2V1

 

S2V2

 

S2V3

 

S2V4

 

S2V5

 

 

 

 

Layout Plan Attachment
Treatment

Treatment Details:

S1V1 = Contaminated Soil (S1) + Fenugreek (V1)

S1V2 = Contaminated Soil (S1) + Spinach (V2)

S1V3 = Contaminated Soil (S1) + Coriander (V3)

S1V4 = Contaminated Soil (S1) + Amaranthus (V4)

S1V5 = Contaminated Soil (S1) + Dill leaves (V5)

S2V1 = Non-Contaminated Soil (S2)  + Fenugreek (V1)

S2V2 = Non-Contaminated Soil (S2)  + Spinach (V2)

S2V3 = Non-Contaminated Soil (S2)  + Coriander (V3)

S2V4 = Non-Contaminated Soil (S2)  + Amaranthus (V4)

S2V5 = Non-Contaminated Soil (S2) + Dill leaves (V5)

Treatment Attachment
(NAU-EMP-2019-000681) SURESHKUMAR MAGANBHAI BAMBHANEEYA sureshsoilscience@nau.in 7874206663 07-01-2022
Active
(NAU-EMP-2015-001747) NARENDRAKUMAR HAMIRBHAI GARANIYA narendra.biochem@nau.in 9725456394 03/03/2020
Active
(NAU-EMP-2013-000642) KIRITKUMAR HIRALAL PATEL khpatel@nau.in 9428354624 19/02/2022
Active
(NAU-EMP-2015-000666) SWAPNIL PRASADRAO DESHMUKH swapnildeshmukh@nau.in 9510146338 19/02/2022
Active
Spinach Spinach
Amaranthus Amaranthus