Experiment code 18.8.3.12
Experiment Title BIOCHEMICAL AND MOLECULAR CHARACTERIZATION OF CRY1AC GENOTYPES OF COTTON (Gossypium hirsutum L.)
Research Type Lab Research
Experiment Background Cotton is the most important commercial crop of India, often referred as the White Gold. In India, about 95% area in cotton is under the Bollgard-II. This technology has contributed largely to cotton production and productivity in India. As hybrids are inherently designed to perform best under high input conditions and most the hybrids available in India are of long duration and are not ideally suited for rain-fed tracts. Adaption Bt technology only in hybrids led to yield stagnation, doubling of fertilizer use and resurgence of insecticide usage over the past 6-7 years is primarily a growing concern. The use of Bt technology in elite varieties of cotton would harness full potential of technology. The seed cost of Bt varieties is much lower than the cost of hybrid seed. Thus, a Bt variety is a cheaper alternative. At our research center we have selected the Cry1Ac lines from the advanced backcross generations and continuously selfed them in on order generate homozygous stable lines of Bt varieties. Further characterization of these Bt cotton varieties biochemically and for Cry1Ac protein at different growth stages along with genetic diversity using molecular maker would pave the way for identification of promising Bt cotton varieties.
Experiment Group Basic Science
Unit Type (01)RESEARCH UNIT
Unit (46)MAIN COTTON RESEARCH STATION (SURAT)
Department (269)Main Cotton Research Station Surat
BudgetHead (313/12014/00)313/01/REG/00087
Objective
  1. To estimate biochemical parameters at different growth stages
  2. To estimate the Cry1Ac protein at different growth stages
  3. To estimate genetic diversity among cotton  genotypes using molecular markers
Season Kharif
Location Unit Type (01)RESEARCH UNIT
Location Unit (46)MAIN COTTON RESEARCH STATION (SURAT)
Location Department (269)Main Cotton Research Station Surat
Plot No Not applicable
PI Name (NAU-EMP-2012-000569)RAJKUMAR BABURAO KATAGI
PI Email rajkumar@nau.in
PI Mobile 8511835498
Year of Approval 2022
Commencement Year 2022
Completion Year 2024
Design of Experiment

CRD

Crop Spacing (cm x cm)

Not Applicable 

Gross Plot (m x m) Not Applicable 
Net Plot (m x m) Not Applicable 
Total Experiment Area (m2) Not Applicable 
Plot History Last Three Year

Not Applicable 

Initial Soil Sample Analysis Report

Not Applicable 

Initial Soil Sample Analysis Report Attachment Attachment Not Available!
Layout Plan

Not Applicable 

Layout Plan Attachment Attachment Not Available!
Treatment

Sr. No

Name of Entry

Sr. No

Name of Entry

1

GPC 9 Cry1Ac

6

GISV-267 Cry1Ac

2

GPC 19 Cry1Ac

7

GISV-272 Cry1Ac

3

GPC 28 Cry1Ac

8

GISV-312 Cry1Ac

4

GPC 31 Cry1Ac

9

G. Cot 10 Cry1Ac

5

BC-68-2 Cry1Ac

10

G. Cot.10 NBT

Treatment Attachment Attachment Not Available!
(NAU-EMP-2012-000569) RAJKUMAR BABURAO KATAGI rajkumar@nau.in 8511835498 06-08-2022
Active
(NAU-EMP-2015-000565) PREETIBEN RAMANLAL PARMAR prparmar@nau.in 9824144640 01/08/2022
Active
(NAU-EMP-1995-000586) DIPAK HIRALAL PATEL dhpatel@nau.in 9725001521 01/08/2022
Active
(NAU-EMP-2015-000593) HITESH RANCHHODBHAI RAMANI hrramani@nau.in 9998380285 01/08/2022
Active
Cotton Different varieties of Bt Cotton