Experiment code 18.8.3.13
Experiment Title Metagenomic analysis of flooded rice ecosystem under climate change resilience
Research Type Departmental Research
Experiment Background Methanogens and methanotrophs seem worthy of study as the organisms directly responsible for the emission of methane from soil with global emission rate from paddy fields at 60 Tg/yr. The major pathways of CH4 production in flooded soils are the reduction of CO2 with H2, with fatty acids or alcohols as hydrogen donor, and the transmethylation of acetic acid or methanol by methane-producing bacteria. The global warming potential of methane is 21 times that of carbon dioxide. Namely, compared in equimolecular terms, the greenhouse effect of methane is 21 times greater than that of carbon dioxide. As a result of these studies, some solutions are expected to be developed to reduce methanogenic activity or motivate oxidation of methane by methanotrophs to reduce the methane emission from flooded soils, especially rice fields.
Experiment Group Basic Science
Unit Type (02)EDUCATION UNIT
Unit (61)COLLEGE OF FORESTRY (NAU, NAVSARI)
Department (2364)BASIC SCIENCE AND HUMANITIES,COF, NAU, NAVSARI
BudgetHead (352/12019/00)352/01/REG/04040
Objective
  • To identify the soil microbial community by metagenomic profiling
  • To compare the  microbial diversity present over environment with respect to methane emission
Season Annual
Location Unit Type (02)EDUCATION UNIT
Location Unit (61)COLLEGE OF FORESTRY (NAU, NAVSARI)
Location Department (2364)BASIC SCIENCE AND HUMANITIES,COF, NAU, NAVSARI
Plot No Rice field of NAU
PI Name (NAU-EMP-2010-000958)VIPULKUMAR BHARATBHAI PAREKH
PI Email vipulbiotech@nau.in
PI Mobile 9825992766
Year of Approval 2022
Commencement Year 2023
Completion Year 2025
Design of Experiment
  • Sampling site: N.A.U farm.
  • Site for sampling: Three site
  • Sample collection time:

     1. Before Transplanting (Before puddling)

  2. One month after transplanting (submerged soil)

  3. Two month after transplanting

Sample size: 3 Site X 3-time factor = 6

  • Replications: 3
  • Design: FCRD

Molecular work: Forest Biotechnology Laboratory

Crop Spacing (cm x cm)

20x15 

Gross Plot (m x m) NA
Net Plot (m x m) NA
Total Experiment Area (m2) NA
Plot History Last Three Year

NA

Initial Soil Sample Analysis Report

NA

Initial Soil Sample Analysis Report Attachment Attachment Not Available!
Layout Plan
  • Sampling site: N.A.U farm.
  • Site for sampling: Three site
  • Sample collection time:

     1. Before Transplanting (Before puddling)

  2. One month after transplanting (submerged soil)

  3. Two month after transplanting

Sample size: 3 Site X 3-time factor = 6

  • Replications: 3
  • Design: FCRD

Molecular work: Forest Biotechnology Laboratory

Layout Plan Attachment Attachment Not Available!
Treatment
  • Sampling site: N.A.U farm.
  • Site for sampling: Three site
  • Sample collection time:

     1. Before Transplanting (Before puddling)

  2. One month after transplanting (submerged soil)

  3. Two month after transplanting

Sample size: 3 Site X 3-time factor = 6

  • Replications: 3
  • Design: FCRD

Molecular work: Forest Biotechnology Laboratory

Observations:

  • DNA quantification and quality analysis
  • DNA Sequence data acquisition by  16s r DNA / NGS platform (As per available resources )
  • Data analysis on Bioinformatics platforms
  • SOM
  • Available Nutrients: N, P, K, S, Fe and Zn
  • Weather parameters: Temperature, humidity, sunshine hours etc.
  • Methane flux at the time of soil sampling

 

Treatment Attachment Attachment Not Available!
(NAU-EMP-2010-000958) VIPULKUMAR BHARATBHAI PATEL vipulbiotech@nau.in 9825992766 21-01-2023
Active
(NAU-EMP-2013-000949) SHAILENDRASINH VIJAYSINH VIYOL sviyol@nau.in 9898842199 01/08/2022
Active
Rice