Experiment code 18.8.3.9
Experiment Title Standardization of extraction protocol for curcumin and their stability assay
Research Type Lab Research
Experiment Background Turmeric (Curcuma longa) is a medicinal plant used in ayurveda, unani and siddha medicine as home remedy for various diseases. Curuma longa botanically related to ginger (zingiberaceae family), is a perennial plant having a short stem with large oblong leaf and bears ovate, pyriform or oblong rhizomes, which are often branched and brownish- yellow in colour .Component of turmeric are named Curcuminoids, which include namely curcumin, demethoxycurcumin, and bisdemethoxycurcumin. Curcumin, the main yellow bioactive component of turmeric have been shown to have a wide spectrum of biological actions (Chattopadhyay et al, 2004).Turmeric is used as a food additive, preservative and colouring agent in Asian countries. It is also considered as auspicious and is a part of religious rituals . In traditional Indian medicine uses turmeric powder for the treatment of biliary disorders, anorexia, coryza, cough, diabeticwounds, hepaticdisorder, rheumatism, sinusitis (Amman, H.P et al 1991). The colouring principle of turmeric is the main component of this part and is responsible for the anti inflammatory property. Curcumin (diferuloyl methane), the main yellow bioactive component of turmeric has been shown to have a wide spectrum of biological actions (Chottopadhyay et al ,2004).
Experiment Group Basic Science
Unit Type (02)EDUCATION UNIT
Unit (20)ASPEE SHAKILAM BIOTECHNOLOGY INSTITUTE (SURAT)
Department (312)ASPEE Shakilam Biotechnology Institute
BudgetHead (339/12248/00)339/03/REG/01870
Objective

1. To extract Curcumin form Curuma longa

2. To check Purity and stability of extracted curcumin

Season Not season specific
Location Unit Type (02)EDUCATION UNIT
Location Unit (20)ASPEE SHAKILAM BIOTECHNOLOGY INSTITUTE (SURAT)
Location Department (312)ASPEE Shakilam Biotechnology Institute
Plot No Not Applicable
PI Name (NAU-EMP-2013-000485)HARSURBHAI MANGLUBHAI JAJDA
PI Email hmjajda@nau.in
PI Mobile 8128699171
Year of Approval 2022
Commencement Year 2022
Completion Year 2025
Design of Experiment

 1 gram of turmeric from Curcuma longa was weighed accurately and extracted using different polar solvents (Methanol, Ethanol, Petrolium ether and Acetone). The resultants were centrifuged at 10,000 rpm for 5 minutes at room temperature and the supernatants were collected. This procedure was repeated until the residue become colourless. The supernatant was collected and kept in dark overnight. The dried extract was weighed, yield was calculated and solubilised in DMSO and stored. The stored material will be used to check stability of curcumin.

  1. Yield of curcumin
  2. Antioxidant activity of curcumin after every 15 days upto 3 month (DPPH assay, FRAP assay )
  3. Total Phenolic content
  4. Antibacterial activity of extracts
  5. Spectral analysis of extract(400nm to 800 nm)
  6. To check the purity of extracted curcumin by HPLC
Crop Spacing (cm x cm)

Not applicable

Gross Plot (m x m) Not applicable
Net Plot (m x m) Not applicable
Total Experiment Area (m2) Not applicable
Plot History Last Three Year

Not applicable

Initial Soil Sample Analysis Report

Not applicable

Initial Soil Sample Analysis Report Attachment Attachment Not Available!
Layout Plan

Not applicable

Layout Plan Attachment Attachment Not Available!
Treatment
  1. Yield of curcumin
  2. Antioxidant activity of curcumin after every 15 days upto 3 month (DPPH assay, FRAP assay )
  3. Total Phenolic content
  4. Antibacterial activity of extracts
  5. Spectral analysis of extract(400nm to 800 nm)
  6. To check the purity of extracted curcumin by HPLC
Treatment Attachment Attachment Not Available!
(NAU-EMP-2013-000485) HARSURBHAI MANGLUBHAI JAJDA hmjajda@nau.in 8128699171 21-01-2023
Active
(NAU-EMP-2015-000481) HIMANSHU DHIRAJLAL BHIMANI himanshubhimani@nau.in 9426422426 18/03/2022
Active
Turmeric GNT-2