Development of colorimetric sensors for the pesticides in agricultural produces
Research Type
Lab Research
Experiment Background
Pesticides act as toxic compounds to all living organisms and hence used to control the insects, weeds, nematodes and other various pests. The pesticides can be carcinogenic in nature, which can produce disorders in bone marrow and nervous system and can sometimes cause infertility and respiratory diseases (Sci. Rep., 2020). Therefore, the trace level determination of pesticides is an important issue to be addressed. Generally, the use conventional techniques like HPLC, AAS, GC, GF-AAS etc is limited as they often required highly sophisticated instrumentation and tedious sample preparation. Recently, metal nanoparticles have garnered a significant interest in various fields of science because of their size dependent optical properties, and they have shown a great impact in improving the analytical performance of UV-visible spectrometry, just like as sophisticated instruments. Surface modified metal nanoparticles (Au, Ag and Cu) have the potential to establish miniaturized colorimetric methods for assaying of wide variety molecules, offering the quantification of trace level target analytes via the color changes, which could be noticed with naked-eye. The interface between the ligand chemistry of metal nanoparticles and trace level pesticides is where the specific covalent and non-covalent interactions occur; therefore, a significant color change occurs in the metal nanoparticles via the aggregation of metal nanoparticles induced by pesticides. In this review, the role of ligand chemistry on the surfaces of metal nanoparticles (Au, Ag and Cu) for colorimetric detection of various pesticides (Food Chem., 2021).
Experiment Group
Basic Science
Unit Type
(02)EDUCATION UNIT
Unit
(20)ASPEE SHAKILAM BIOTECHNOLOGY INSTITUTE (SURAT)
Department
(312)ASPEE Shakilam Biotechnology Institute
BudgetHead
(339/12248/00)339/03/REG/01870
Objective
1. To develop the nanoparticle based colorimetric probe. 2. To evaluate selectivity and sensitivity of the developed probe.
Season
Not season specific
Location Unit Type
(02)EDUCATION UNIT
Location Unit
(20)ASPEE SHAKILAM BIOTECHNOLOGY INSTITUTE (SURAT)
1. Synthesis of nanoparticles (Au or Ag). 2. Functionalization of nanoparticles to develop the colorimetric probe. 3. Selectivity of colorimetric probe towards pesticides (Carbamate and Organophosphate). 4. Sensitivity of the colorimetric probe 5. Interference study 6. Application of colorimetric probe for real sample analysis (Mango and Okra)
Crop Spacing (cm x cm)
Not Applicable
Gross Plot (m x m)
Not Applicable
Net Plot (m x m)
Not Applicable
Total Experiment Area (m2)
Not Applicable
Plot History Last Three Year
Not Applicable
Initial Soil Sample Analysis Report
Not Applicable
Initial Soil Sample Analysis Report Attachment
Attachment Not Available!
Layout Plan
Not Applicable
Layout Plan Attachment
Attachment Not Available!
Treatment
Observations to be recorded: 1. Characterization of functionalized nanoparticles 2. Selectivity study 3. Sensitivity study 4. Effect of pH 5. Real sample analysis (% Recovery and RSD)