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(a) Contamination Control Treatments
C1 - Mercuric Chloride (0.1%) for 3 Min
C2 - Mercuric Chloride (0.1%) for 4 Min
C3 - Mercuric Chloride (0.1%) for 5 Min
C4 - Absolute Alcohol (70%) for 30 Sec + Mercuric Chloride (0.1%) for 3 Min
C5 - Absolute Alcohol (70%) for 30 Sec + Mercuric Chloride (0.1%) for 4 Min
C6 - Absolute Alcohol (70%) for 30 Sec + Mercuric Chloride (0.1%) for 5 Min
(b) Shoot Multiplication Treatments
T1 - MS + 0.5 mg/l BAP
T2 - MS + 1.0 mg/l BAP
T3 - MS + 2.0 mg/l BAP
T4 - MS + 3.0mg/l BAP
T5 - MS + 4.0 mg/l BAP
(c) Rooting Treatments
T1 – MS + 1 mg/l IBA + 0.25 % Activated charcoal
T2 – MS + 0.1% IBA+ 2% Sucrose
T3 – MS + 1 mg/l IBA + 1 mg/l NAA + 2% Sucrose
T4 – MS + 1 mg/l IBA + 1 mg/l NAA
T5 – MS + 1.5 mg/l NAA + 3 mg/l IBA + 2% Sucrose
T6 – MS + 1 mg/l NAA + 0.3% Activated charcoal
T7 - MS + 20 mg/l IBA
T8 - MS + 0.5mg/l IBA
T9 – MS + 1 mg/l BAP + 3 mg/l NAA
T10 – MS + 1.8 mg/l NAA + 10 mg/l Coumarin
T11 – MS + 1.8 mg/l NAA
T12 – MS + 1 mg/l BAP + 1 mg/l NAA + 3% Activated charcoal
T13 – MS + 3 mg/l NAA+ 2% Sucrose
T14 – MS + 0.2 mg/l NAA + 0.2 mg/l IBA + 10 mg/l Coumarin
T15 - ½ MS + 1.8 mg/l NAA + 2 mg/l IBA + 10 mg/l Coumarin + 2 % Sucrose
T16 - ½ MS + 0.5 mg/l IBA + 0.5 mg/l NAA + 2 % Sucrose
T17 - ½ MS + 1 mg/l IBA + 0.5 mg/l NAA + 2 % Sucrose
T18 - ½ MS + 0.5 mg/l NAA
T19 - ½ MS + 1 mg/l NAA
T20 - ½ MS + 1 mg/l IBA
T21 - ½ MS + 2 mg/l IBA
T22 - MS + 3 mg/l IBA
T23 - MS + 4 mg/l IBA
T24 - MS + 5 mg/l IBA
T25 - ½ MS + 3 mg/l IBA
T26 - ½ MS + 3 mg/l IBA + 3 mg/l NAA + 2% Sucrose
T27 - ½ MS + 3 mg/l IBA + 10 mg/l Coumarin
T28 - ½ MS + 0.2 mg/l IB
T29 - ½ MS + 1 mg/l IBA + 1 mg/l NAA
T30 - ½ MS + 2 mg/l IBA + 1 mg/l NAA
T31 - ½ MS + 5 mg/l IBA + 1 mg/l NAA
T32 - MS + 8µM BAP + 1µM NAA + 100 µM IBA
T33 - ½ MS + 8µM BAP + 1µM NAA + 100 µM IBA
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