Melia dubia is a imperative multipurpose tree used in bio energy production, paper and pulp industry, plywood industry as well as having medicinal uses. M. dubia belongs to the Meliaceae family. It is significantly distributed in India, Iran, Pakistan, Argentina, Brazil, Bermuda, China, Australia, and Malaysia. All the parts of M. dubia were used as a medicine and various kinds of treatment such as for wound healing, treatment of skin, against insect pests, and gastro intestinal infections. It has a antibacterial, antifungal, antiviral, hypoglycaemic, antineoplastic, antileprosy and antihelmintic properties. Conventional propagation of M. dubia shows poor success due to hard stony seed coat. Therefore to cope up with the elevated demand of various industries, in vitro regeneration is the possible approach to prepare true to type plantlets. Therefore it is an immense requisite to develop an optimized protocol for micropropagation of M. dubia.
Experiment Group
Basic Science
Unit Type
(02)EDUCATION UNIT
Unit
(20)ASPEE SHAKILAM BIOTECHNOLOGY INSTITUTE (SURAT)
Department
(312)ASPEE Shakilam Biotechnology Institute
BudgetHead
(339/12248/00)339/03/REG/01870
Objective
1. To standardize establishment media for node
2. To standardize multiplication media
3. To standardize the media for rooting of in vitro regenerated shoots
4. To standardize pot mixture for acclimatization of in vitro regenerated plantlets
Season
Not season specific
Location Unit Type
(02)EDUCATION UNIT
Location Unit
(20)ASPEE SHAKILAM BIOTECHNOLOGY INSTITUTE (SURAT)
Location Department
(312)ASPEE Shakilam Biotechnology Institute
Plot No
Not applicable
PI Name
(NAU-EMP-2015-000493)SWATI KIRANBHAI PATEL
PI Email
swati_20188@nau.in
PI Mobile
9879688080
Year of Approval
2021
Commencement Year
2021
Completion Year
2024
Design of Experiment
• CRD (Completely Randomized Design) with 10 bottles per treatment and 3 repetition
• 5 repetitions for root induction and acclimatization
Crop Spacing (cm x cm)
Not applicable
Gross Plot (m x m)
Not applicable
Net Plot (m x m)
Not applicable
Total Experiment Area (m2)
Not applicable
Plot History Last Three Year
Not applicable
Initial Soil Sample Analysis Report
Not applicable
Initial Soil Sample Analysis Report Attachment
Attachment Not Available!
Layout Plan
Not applicable
Layout Plan Attachment
Attachment Not Available!
Treatment
A) Treatments for shoot establishment
Treatment no. Media
TE1 MS
TE2 MS + 0.1 mg/L BAP
TE3 MS + 0.5 mg/L BAP
TE4 MS + 1.0 mg/L BAP
TE5 MS + 1.5 mg/L BAP
TE6 MS + 2.0 mg/L BAP
TE7 MS + 0.1 mg/L BAP + 0.1 mg/L NAA
TE8 MS + 0.5 mg/L BAP + 0.1 mg/L NAA
TE9 MS + 1.0 mg/L BAP + 0.2 mg/L NAA
TE10 MS + 1.5 mg/L BAP + 0.2 mg/L NAA
TE11 MS+ 2.0 mg/L BAP + 0.5mg/L NAA
B) Treatments for shoot multiplication
Treatment no. Media
TM1 MS + 0.25 mg/L BAP
TM2 MS + 0.5 mg/L BAP
TM3 MS + 1.0 mg/L BAP
TM4 MS + 0.25 mg/L BAP + 2.5 mg/L GA3
TM5 MS + 0.5 mg/L BAP + 2.5 mg/L GA3
TM6 MS + 1.0 mg/L BAP + 2.5 mg/L GA3
TM7 MS + 0.25 mg/L BAP + 30 mg/L Adenine sulphate
TM8 MS + 0.5 mg/L BAP + 30 mg/L Adenine sulphate
TM9 MS + 1.0 mg/L BAP + 30 mg/L Adenine sulphate
TM10 MS + 0.25 mg/L BAP + 2.5 mg/L GA3 + 30 mg/L Adenine sulphate
TM11 MS + 0.5 mg/L BAP + 2.5 mg/L GA3 + 30 mg/L Adenine sulphate
TM12 MS + 1.0 mg/L BAP + 2.5 mg/L GA3 + 30 mg/L Adenine sulphate
C) Treatments for root induction of in vitro regenerated shoots
Treatment no. Media
TR1 ½ MS
TR2 ½ MS + 0.1 mg/L IBA
TR3 ½ MS + 0.5 mg/L IBA
TR4 ½ MS + 1.0 mg/L IBA
TR5 MS + 0.1 mg/L IBA
TR6 MS + 0.5 mg/L IBA
TR7 MS + 1.0 mg/L IBA
D) Treatments for acclimatization of in vitro regenerated plantlets
Treatment no. Pot Mixture
TH1 Soil: vermicompost (1:1)
TH2 Soil: vermicompost: cocopeat (1:1:1)
TH3 Soil: vermicompost(1:2)
TH4 Soil: cocopeat: vermicompost (1:2:1)
TH5 Sand: Soil: vermicompost (1:1:1)
TH6 Cocopeat: vermicopost:sand (1:1:1)